Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 1 – Specific expression of PSGR in human prostate tissues using in situ hybridization. (A) Overexpression of PSGR in human prostate malignant tissues with different clinical stages and Gleason scores. (A-a) Gleason score 7, clinical stage II, T2N0M0. (A-b) Gleason score 4, clinical stage III, T3N0M0. (A-c) Gleason score 9, clinical stage IV, T4NXM1. (A-d) Gleason score 9, clinical stage IV, T3N1M0. (B) PSGR expression in prostate PIN and normal tissue. Strong PSGR expression is noted in varied stages of PIN (arrow) samples, whereas very low amount of PSGR transcripts are evident in adjacent normal prostate tissues (N, arrow). (B-a) Expression of PSGR in tufting pattern of PIN and normal prostate tissue. (B-b) PSGR expression in the micropapillary pattern of PIN lesion. (B-c and B-d) In situ hybridization of PSGR expression using PSGR sense probe as a control. (C) Expression of PSGR in nonprostate malignant tissues. No PSGR expression was detected in other malignant tissues. (C-a) Breast cancer tissue. (C-b) Lung cancer. (C-c) Stomach cancer. (C-d) Uterine cancer tissue.

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Expressing, In Situ Hybridization, Over Expression, Control

Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 2 – The expression of PSGR mRNA level (copies/103 -ac- tin) in normal prostate tissue (normal, 19), BPH (8) and PCa (57) determined by real-time RT-PCR method. The expression level of PSGR mRNA detected in the prostate tumor tissue is significantly higher than that from the normal prostate tissue and BPH tissue (p 0.001, Mann-Whitney U-test). No significant difference was detected for the expression of PSGR between normal prostate and BPH (p 0.05, Mann-Whitney U-test).

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Expressing, Quantitative RT-PCR, MANN-WHITNEY

Journal: International journal of cancer

Article Title: Increased expression of prostate-specific G-protein-coupled receptor in human prostate intraepithelial neoplasia and prostate cancers.

doi: 10.1002/ijc.20635

Figure Lengend Snippet: FIGURE 3 – ROC for PSGR real-time PCR assay. The assay has high-level sensitivity and specificity for discriminating benign prostate tissue from malignant prostate tissue in laboratory (AUC 0.902 0.032).

Article Snippet: In situ hybridization of PSGR in prostate tissues Tissue slides containing 56 prostate tumors and 11 nonprostate tumors of varied clinical stages, 6 normal prostate tissues and 11 normal nonprostate tissues embedded in paraffin were bought from IMGENEX.

Techniques: Real-time Polymerase Chain Reaction